#Design based stereology manual#
#Design based stereology software#

Hands-on Workshop, Dates to-be-determined when it possible to travel again

Remain productive in the lab while participatingВ in the workshop.

Save the time, expense, and uncertainty ofВ traveling to Chicago.

Take advantage of subsequent individualВ consultation sessions on your research needs.

Participate in Zoom-based Discussion Groups.

Attend on-line half-day interactive, live webinars (download the syllabus for topics).

SummaryВ of the Workshops: Virtual Workshop,В March 7-11, 2022

Investigators new to histological analysis who need a rapid and complete introduction to these techniques.

Young investigators who need to incorporate these analytical approaches into their labs.

Postdoctoral fellows who would benefit from a comprehensive introduction to microscopy and stereology.

Graduate students who need to use modern microscopy or stereology in their projects.

See August 2020 virtual workshop Survey Monkey results

Post-workshop individual consultation onВ your research objectives and approachesДownload the Workshop ProgramВ (pdf format) here.

New topics in cleared tissue, automated confocal stereology, and artificial intelligence.

Unbiased study design and use of quantitative histology/ stereology.

Proper imaging approaches and ethics, scientific image construction including a Photoshop tutorial.

Background and theory of sample preparation and in microscope design and use.

Histomorphometry optimizes throughput and comprehensive analysis but requires user thresholding.The nextВ Workshop is offered March 7-11, 2022В as a Virtual Workshop

#Design based stereology manual#

However, the light microscopy, but not electron microscopy, stereological analysis yielded higher nerve fiber area compared to histomorphometry or manual measurement.Д¬onclusion: Both methods measure similar fiber number and overall nerve fiber area however, stereology with light microscopy quantified higher fiber area. Both histomorphometry and stereological peripheral nerve analyses were performed on all specimens by two blinded and independent investigators who quantified nerve fiber count, fiber width, density, and related distribution parameters.Д¬omparison with existing methods: Histomorphometry and stereological analysis provided similar outcomes in nerve fiber number and total nerve area. From each animal, sciatic nerve specimens were fixed, stained, and sectioned for analysis by light and electron microscopy. Results: Sciatic nerves were harvested from 6 male Lewis rats, aged 8вЂ“12 weeks for comprehensive analysis of 6 nerve specimens. New Method: Stereological analysis was compared to histomorphometry with binary image analysis on uninjured sciatic nerves to determine nerve fiber number, nerve area, neural density, and fiber distribution.

This study investigated the reliability of design-based stereology versus semi-automated binary imaging histomorphometry for assessing healthy peripheral nerve characteristics. While these methods of analysis are complementary, no study to date has systematically compared both approaches in peripheral nerve. Moore, Eva L Feldman, Susan E Mackinnon, Michael J Brenner AbstractД«ackground: Stereology and histomorphometry are widely used by investigators to quantify nerve characteristics in normal and pathological states, including nerve injury and regeneration. Adding other measurement parameters, such as circular diameter, aspect ratio, or roughness is easily done within the software.ДЄuthors: Daniel A Hunter, Deng Pan, Matthew D Wood, Alison K.

#Design based stereology software#

Combining the two colors into another binary plane, the software could also measure the total fiber width and area of axon + myelin. The software was able to measure the area of each component вЂ“ myelin (blue) and axon (magenta). The role of Clemex software in this studyД¬lemex Vision PE was used to detect nerves separately from background non nerve profiles.

1 Nerve profiles detected in blue (myelin) and magenta (axon) using Clemex Vision PE software.